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Objective :To study influence of folic acid ,vitamin B6 and vitamin B12 on vascular endothelial function serum level of homocysteine (Hcy) and living quality in hypertensive patients with acute cerebral infraction .Meth‐ods :A total of 92 hypertensive patients with acute cerebral infraction treated in our hospital from Feb 2016 to Oct 2017 were randomly and equally divided into routine treatment group and combined treatment group (received folic acid ,vitamin B6 and vitamin B12 based on routine treatment group ).Two groups were both treated for 12 weeks Flow‐mediated dilation (FMD) ,serum levels of Hcy and nitric oxide (NO ) , United States National Institutes of Health Stroke Score (NIHSS) score and Barthel index score before and after treatment were compared between two groups.Results :Compared with before treatment ,after treatment ,there were significant reductions in serum level of Hcy and NIHSS score ,and significant rise in Barthel index score in two groups ,and there was significant rise in serum level of NO [ (50. 20 ± 11.18) μmol／L vs.(66. 53 ± 10. 66) μmol／L] in combined treatment group , P＜0. 01 all.Before and after treatment , there were no significant difference in FMD in two groups , and no significant difference in serum level of NO in routine treatment group , P＞0.05 all.Compare with routine treatment group after treatment ,there were significant reductions in serum level of Hcy [ (21.58 ± 2.24 ) μmol／L vs.(17.27 ± 2.19) μmol／L] and NIHSS score [(20. 39 ± 3. 19) scores vs .(16.12 ± 1.15) scores] ,and significant rise in NO level [ (53.76 ± 11. 67) μmol／L比(66.53 ± 10. 66) μmol／L] ,Barthel index score [ (62.27 ± 20.58) scores vs .(79. 58 ± 21. 24) scores] in combined treatment group , P＝0.001 all.Conclusion :Folic acid ,vitamin B6 and vitamin B12 can effectively improve vascular endothelial function ,reduce serum level of Hcy ,increase serum level of NO ,improve neurological function and activities of living quality ,which is worth extending .
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the relationship between T cell subsets and clinical data.</p><p><b>METHODS</b>mononuclear cells were collected from 103 patients with acute leukemia (AL) and 28 healthy volunteers, and percentage changes of CD3CD4, CD3CD8 and CD4 CD25 Foxp3 cell subsets were assayed by flow cytometory. Relationship between the T subsets and clinical features of the patients was analyzed.</p><p><b>RESULTS</b>Ratio of CD3 T cells decreased more significantly in patients with >50% blast cells than that in patients with <50% blast cells, while the ratio of Treg between the 2 groups was not significantly different. Treg increased more statistically significantly in the patients with CD34 leukemia cell than that with CD34 leukemia cells. In constrast to the relationship between prognosis and immune cells in the patients from 3 groups (low, intermediate and high-risk group) it was found that Treg cells increased more significantly in high-risk group than that in low-risk group. By continuously monitoring immune cells in 18 patients, it was found that Treg cells gradually increased during the first 3 courses of chemotherapy, then began to decreased in the 4th course, finally approached gradually to the normal value in the 6th course, and this change correlated with the clinical remission after chemotherapy. Treg cell number in the patients with AL was significantly higher than that in healthy controls, and Treg cell number during the onset and recurrence was significantly higher than that in the period of complete remission (continuous remission for over 6 months). Compared with the changes of immune cell number between different types of disease, it was found that Treg cells were increased more significantly in acute myeloid leukemia (AML) than that in acute lymphoblastic leukemia (ALL). Proportion of Treg cells, Treg/CD4 decreased more significantly after the 1st course of chemotherapy in the group with complete remission (CR) than that in the group without CR. The complete remission rate and recurrence rate were 68.9% and 20% respectively in the group with >10% Treg cells, while the complete remission rate and recurrence rate were 85.7% and 7.69% respectively in the group with.<10% Treg cells. In comparison of the 6 recurrent patients with 32 patients with sustained CR, it was found that the ratio of Treg cells and Treg/CD4 was increased more significantly in the patients with relapse than that with CR and in control group.</p><p><b>CONCLUSION</b>Dynamic change of Treg cells in the peripheral blood was closely related with clinical feature, recurrence and prognosis in the patients with acute leukemia.</p>
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<p><b>OBJECTIVE</b>To explore the mechanism of electroacupuncture(EA) at "Fenglong" (ST 40) in hyperlipidemia (HLP) rats.</p><p><b>METHODS</b>Forty health SD rats were randomly divided into a normal control group (group A), a high fat forage feed group (group B) and a high fat forage feed treatment group (group C), a high fat forage + normal forage feed group (group D) and a high fat forage + normal forage feed treatment group (group E), eight rats in each group. EA was applied at "Fenglong" (ST 40) for the rats in group C and group E, once daily. After treatment of 30 days, blood lipid levels of rats, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) in plasma were tested. Real time polymerase chain reaction (RT-PCR) and Western Blotting were applied to detect the gene expression changes of the contents of ATP-binding cassette transporter A1 (ABCA1), peroxisome proliferator-activated receptor alpha (PPARalpha), liver X receptor alpha (LXR-alpha) and retinoid X receptor alpha (RXR-alpha) in liver tissue of rats.</p><p><b>RESULTS</b>Compared with group A, the contents of TC, LDL-C were significantly elevated in group B and group D (all P < 0.01); compared with group B, above indices were significantly decreased in group D (all P < 0.01). After the treatment of EA at "Fenglong"(ST 40), the contents of TC, LDL-C were significantly decreased (all P < 0.01), and the contents of TG, HDL-C did not change obviously (all P > 0.05). Compared with group A, the mRNA and protein contents of ABCA1, PPARalpha, LXR-alpha and RXR-alpha were decreased obviously in group B and group D (all P < 0.01). But compared with group B, the above indices were decreased in the group D. There were signficantly increasing in the protein content of ABCA1, PPARalpha, RXR-alpha and LXR-alpha mRNA after the treatment of EA (all P < 0.05).</p><p><b>CONCLUSION</b>EA at "Fenglong" (ST 40) has some therapeutic effect on decrease the content of TC, LDL-C in rats of hyperlipemia and improve the gene expression of ABCA1, PPARalpha, LXR-alpha and RXR-alpha mRNA so as to promote reverse cholesterol transport.</p>